Cerebrospinal fluid‑contacting nucleus, abbreviated as CSF‑contacting nucleus is a unique nucleus which was originally discovered and named by the author's research team in the world. Significantly different from the known nuclei, its soma is located in the brain parenchyma and protrudes in the cerebrospinal fluid, which is responsible for information transmission between the brain and cerebrospinal fluid. The study lasted more than 30 years and involved more than 100 participants. A series of achievements have been achieved. In 2010, it won the first prize of the Science and Technology of Jiangsu Province. In this paper, we provide an empirical formula for the coordinates of the central point of the nucleus in rats weighing 90‒400 g, fiber projection between the CSF‑contacting nucleus and 235 nuclei in 24 functional regions of the whole brain and their possible functions. Furthermore, seven references were listed in the appendix, including the modeling method of animals with knock‑out CSF‑contacting nucleus, so as to provide necessary working basis for interested researchers to carry out relevant research.