【Abstract】 Sepsis is a systemic inflammatory syndrome caused by infection, with the core mechanism of immune dysfunction. Macrophages are involved in autophagy, phagocytosis, regulation of inflammatory response and killing microorganisms, and are an important element of non‑specific immunity to the body. It has been found that macrophages play an important role in the immune regulation of sepsis through biological behaviors such as polarization and autophagy. The current paper summarized the research progress on macrophage polarization and autophagy in the pathogenesis of sepsis. Regulating macrophage biological behavior is expected to be a new target for the treatment of sepsis in the future.