Objective To investigate the effect of non‑pharmacological interventions on perioperative anxiety and pain in patients undergoing laparoscopic hysterectomy. Methods A total of 80 patients who underwent elective laparoscopic hysterectomy at the First Hospital of Shanxi Medical University from October 2021 to April 2022 were selected. According to the random number table method, they were divided into two groups (n=40): a routine preoperative visit (control) group and a combined intervention (experimental) group. The control group received routine preoperative visit followed by perioperative treatment and monitoring, while the experimental group received three non‑pharmacological treatments: preoperative visits through online WeChat communication, music intervention and aromatherapy, in addition to those in the control group. Both groups were compared for mean arterial pressure (MAP), heart rate, Self‑rating Anxiety Scale (SAS) scores, Visual Analogue Scale (VAS) scores and interleukin‑6 (IL‑6) levels at the preoperative visit (T0), after entering the operating room (T1) and at postoperative 4 h (T2). The two groups were also compared for the General Well‑being Scale (GWB) scores before discharge (T3). Results Compared with the control group, the experimental group showed decreases in MAP, heart rate, SAS scores, and IL‑6 levels at T1 and T2 (P<0.05), and decrease in VAS scores at T2 (P<0.05). Compared with those at T0, the control group presented increases in MAP, heart rate and IL‑6 levels at T1 and T2 (P<0.05), increases in SAS scores and VAS scores at T1 (P<0.05); while the experimental group showed increases in heart rate at T1, increases in MAP and heart rate at T2 (P<0.05), decreases in SAS scores at T2 (P<0.05), and increases in IL‑6 levels at T1 and T2 (P<0.05); VAS scores increased at T2 in both groups (P<0.05). Comparison with those at T1, the control group showed decreased MAP and heart rate at T2 (P<0.05), the experimental group presented increased heart rate at T2 (P<0.05); increases in VAS scores and IL‑6 levels (P<0.05) as well as decreases in SAS scores (P<0.05) were seen at T2 in both groups. The GWB score at T3 in the experimental group was higher than that in the control group (P<0.05). Conclusions Based on routine preoperative visit, the three non‑pharmacological interventions can significantly relieve perioperative anxiety and pain, reduce stress, and enhance perioperative comfort in patients undergoing laparoscopic hysterectomy.