Objective: To investigate the effect and the possible mechanism of hydrogen on acute lung function damage in mice with ARDS induced by lipopolysaccharide. Methods: Thirty-two male C57BL/6J mice, 8 to 10 weeks old, body mass 25 to 35 g. The random number table method was used to divide into four groups: control group (group C), LPS group (group L), LPS + hydrogen group (group LH) and hydrogen group (group H), with eight animals in each group. The ARDS model was established by intraperitoneal injection of LPS 10mg/kg in the L and LH groups, Groups C and H were injected with equal amounts of saline intraperitoneally, and 4% hydrogen inhalation was given to the LH and H groups for 12h. HE staining for lung histopathology. Whole-body plethysmography (WBP) system to detect lung function indicators in mice: minute ventilation volume(MV); expiratory flow 50 (EF50); peak expiratory flow (PEF); enhanced pause (Penh). Scattered Doppler flow imaging to detect the right pulmonary microcirculation in mice. ELISA for the determination of inflammatory factors TNF-α and IL-6 in bronchoalveolar lavage fluid (BALF) of mice. Western Blot assay of mouse lung tissue and serum tissue factor (TF) levels. Results: HE staining showed disorganized lung tissue, neutrophil infiltration, erythrocyte leakage and thrombosis, and thickened interstitial lung edema in group L. Compared with group C, MV [(43.09±14.09) ml/min versus (103.94±13.3) ml/min] was significantly decreased in group L mice, EF50 [(0.87±0.29) ml/s versus (2.42±0.46) ml/s] and PEF [(1.75±0.38) ml/s versus (4.57±0.58) ml/s] were significantly lower and Penh [(1.41±0.17) versus (0.59±0.08)] were significantly higher (both P0.05); Right lung blood flow was significantly slower; TNF-α and IL-6 concentrations in BALF were significantly higher (P0.05); lung tissue and serum TF levels were significantly higher (P0.05). Compared with the group L, mice in the group LH had significantly higher MV [(73.05±17.56) ml/min compared to (43.09±14.09) ml/min], significantly higher PEF [(2.65±0.44) ml/s compared to (1.75±0.38) ml/s], and significantly higher Penh [(0.95±0.22) versus (1.41±0.17)] were significantly lower (all P0.05); Right lung blood flow was significantly increased (P0.05); TNF-α and IL-6 concentrations in BALF were significantly decreased (P0.05); and lung tissue and serum TF levels were significantly decreased (P0.05). Conclusion: Hydrogen alleviates acute lung function damage in mice with ARDS induced by lipopolysaccharide, which may be related to the inhibition of TF expression and reduction of inflammatory factor secretion by hydrogen.