【ABSTRACT】 Objective To investigate the influence of 100 Hz electrical stimulation at the interscapular region on rabbit’s cholecystalgia and the expression of ERK1/2 in spinal posterior horn. Methods Rabbits were randomly divided into five groups: control group, 0.9%NS group, formalin group, stimulating interscapular group and no stimulating interscapular group, six rabbits in each group. By using immunocytochemistry, physiological function test and Western blot technique, the variation of RR, HR and the expression of pERK1/2 in spinal posterior horn of rabbits with cholecystalgia were observed. 100Hz electrical stimulation was applied at the interscapular region of rabbits. Results Not only the RR and HR of rabbits with cholecystalgia were significantly decreased, but also there was 54.04% reduction on the expression of pERK1/2 in spinal posterior horn compared with that of formalin group. Conclusion 100 Hz electrical stimulation at interscapular region can alleviate the cholestagia partly and inhibit the activation of ERK1/2 in spinal posterior horn of rabbits.